FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20410748
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1963895296.21867851192911No Hit
NAGTAGAG2032180.9956420999367588No Hit
AAGGAGAG690130.33812087631477294No Hit
AAGTCGAG473090.23178474399860308No Hit
AAGTGGAG469270.22991318103579547No Hit
ACGTAGAG345870.16945483820583154RNA PCR Primer, Index 17 (100% over 8bp)
AGGTAGAG330680.16201268076995512No Hit
AAGTAGCG326110.15977366434586326No Hit
AAGTTGAG323990.15873499589529988No Hit
AAGAAGAG291070.14260623863466443No Hit
AAGTAGGG262810.128760592213475No Hit
AAGTAGAA247370.1211959502905038No Hit
AAGCAGAG240810.11798195734913783No Hit
CAGTAGAG232350.1138370823058518No Hit
GAGTAGAG225230.1103487241133936No Hit
ANGTAGAG221570.1085555512223266No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)