FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20151833
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA32950.082.464481
TTACGCG14450.081.777479
GTGCGAC33550.077.506291
CGCCGAA30800.073.75461
ATAACGC35000.070.53881
TTTGCGC33250.070.516841
CGGTACG30600.070.17871
ACGACCG31100.069.285291
CGAACGC38050.069.1078341
GGCGACT37000.068.305211
AACGCGT26950.066.980123
TAGGCGC39050.066.402851
TTACCCG38750.066.162951
GTTACGC35150.065.999188
AACCGCG38150.065.863271
GCGCTAG30950.065.8450241
TCACGCG31400.064.8769768
GACGCGT30150.064.200431
GCGCGTA36550.063.9502141
TCGCGAT22650.063.05398
Produced by FastQC (version 0.11.5)