FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20147831
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13050.085.547689
CGCCGAA33200.078.5526051
TGCGCGA32050.076.5980151
CGGTACG31300.075.873281
GTGCGAC35150.075.6455151
ACGACCG30650.073.917211
TTTGCGC30400.072.36841
CGAACGC35800.068.9814451
GCGCTAG33550.067.527951
GACGCGT30650.066.7869341
ATAACGC35600.066.504191
TCACGCG30150.064.91948
CCGCTAT40250.064.4317251
TTACCCG38300.064.288541
GCCGGAT37450.064.1915361
ACGCCGA42650.063.538881
TAGGCGC34850.063.3367081
GGCGACT42400.063.0544661
GTTACGC31350.062.4344448
GTACCGT45350.062.3426678
Produced by FastQC (version 0.11.5)