FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20147831
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC68100.0118.699015
CGACGCT69550.0115.703436
CACGACG71100.0113.588654
GACGCTC82550.097.7461247
TACACGA85500.095.436592
CTACACG88050.092.622411
ACGCTCT90150.089.585068
ACACGAC91800.089.2399753
CGCTCTT103650.077.498659
TCTTCCG150800.011.596127510-14
CTTCCGA169800.010.76823210-14
CGATCTA51700.09.59127715-19
TTCCGAT207400.09.01878410-14
TCGCCGG22600.08.9816475145
TCCGATC209900.08.5654515-19
CCGATCT226050.08.23571715-19
GCTCTTC240650.07.489490510-14
GCGTCGG33000.07.0297914145
CGATCTT77700.06.12059715-19
TAACCCT867100.05.93621446
Produced by FastQC (version 0.11.5)