FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21090520
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12950.082.845669
TGCGCGA32550.079.473891
CGCCGAA34600.076.682231
ACGACCG28050.075.145241
GCGCTAG36500.071.479051
GTGCGAC34350.071.232751
CGGTACG32150.070.834661
TTTGCGC32600.070.761181
CGTTGCG16250.070.036777
TTACCCG40300.066.933631
CGAACGC39000.066.897051
TCGTACG38150.066.3153158
GGCGACT40650.065.26951
GCGCGTA35350.065.0479351
CCGCTAT42350.064.389731
GTTACGC32200.064.3862468
AACCGCG39700.063.1185071
GTACCGT46650.062.778758
CCTTACG36650.062.7406431
CGCGTGA39450.062.2107661
Produced by FastQC (version 0.11.5)