FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18939614
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22850.093.542966
ACGACGC25100.085.73345
CACGACG26400.081.7861254
GACGCTC36450.059.4359177
TACACGA38600.056.983332
ACGCTCT41600.052.4248288
CTACACG44900.050.365481
ACACGAC53600.041.3644643
CGCTCTT58950.036.872689
CGTGCGG17901.4886589E-46.0717134145
TAACCCT731100.05.87696656
CTAACCC780500.05.65342575
AACCCTA790450.05.61998371
ACCCTAA812950.05.5988162
CCCTAAC814550.05.57725523
CCTAACC809700.05.50323774
AACGGGG38651.8171704E-95.49122571
TCTTCCG97700.05.473054410-14
GTTCCGA47753.6379788E-125.4625625
CGCAAAG29801.3761637E-65.40290361
Produced by FastQC (version 0.11.5)