FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18767523
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12900.085.983499
TTTGCGC30700.079.17671
TGCGCGA27150.078.237461
GCGCTAG33800.077.313961
CGCCGAA27250.076.878861
ACGACCG28500.073.506981
GTGCGAC32900.072.5511
CGGTACG29650.069.67121
ATAACGC35350.068.761661
TTACCCG35100.067.587721
CGAACGC34700.067.315031
TAGGCGC34650.066.780181
GGCGACT35800.066.2661741
TCGTACG34950.065.13228
GCGCGTA32350.063.8562971
GTACCGT40350.063.7828
GACGCGT28400.063.7418861
AACCGCG38050.063.115031
AACGCGT26050.062.909953
TCACGCG27850.062.2136968
Produced by FastQC (version 0.11.5)