FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17679086
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26900.078.96571
TTTGCGC29000.078.78511
ACGACCG27500.077.773691
CGCCGAA27300.077.006541
CGGTACG29050.074.37781
TTACGCG10350.073.5463949
GCGCTAG30700.071.806931
GTGCGAC30650.071.685911
GGCGACT34200.067.6598361
ATAACGC33900.067.181961
TTACCCG36800.067.0450441
GCGCGTA28250.066.665181
CCGCTAT34900.066.0936051
AACGCGT25900.065.516793
GTACGCT26850.065.0706568
GACGCGT27650.064.415811
CGAACGC32050.063.7716671
TAGGCGC35150.063.7544941
GTACCGT37900.063.6967748
GCCGGAT31700.063.0941471
Produced by FastQC (version 0.11.5)