FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20034598
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11350.084.944069
TGCGCGA30350.081.827881
CGCCGAA30750.077.4082951
GTGCGAC35850.076.879851
TTTGCGC31500.076.267081
ACGACCG28100.072.120121
GCGCTAG34550.071.667341
CGGTACG32650.068.16381
CCGCTAT37600.067.617841
CGAACGC38550.066.907341
ATAACGC37250.065.879161
TTACCCG37400.065.811971
TAGGCGC39700.065.340471
GGCGACT41500.064.637321
GACGCGT31300.064.275931
TCGCGAT24550.064.074858
TCACGCG28350.062.6458668
GTTACGC30600.062.540588
TCCGCGA29800.062.516738
GCCGGAT38650.061.7768251
Produced by FastQC (version 0.11.5)