FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20530768
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10700.080.6273359
CGGTACG31500.077.623321
TTTGCGC33000.075.8644261
GTGCGAC34600.075.731461
TGCGCGA30950.074.050331
CGCCGAA29900.073.233211
TTACCCG39850.071.798561
GCGCTAG33300.071.0164261
ATAACGC37600.069.883181
ACGACCG31650.069.414591
AACCGCG36850.068.136371
GGCGACT40600.067.775591
GTACGCT29050.066.632228
TAGGCGC38100.066.284061
TTCCGCG20450.065.2280047
CCGCTAT41450.064.448761
GTCGGAC31500.064.415771
GTTACGC31250.064.261218
GCCGGAT38400.064.245611
AACGCGT28550.063.7544483
Produced by FastQC (version 0.11.5)