FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21736017
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13400.084.937069
TGCGCGA35050.078.153331
ACGACCG33000.077.4891361
CGCCGAA34800.074.946511
TTTGCGC33750.074.471991
GTGCGAC38600.073.607991
CGGTACG33450.071.219561
TTACCCG44300.069.892861
GCGCTAG36500.067.86311
CGAACGC41650.067.343141
TCGTACG43200.065.11048
GGCGACT42900.064.7016451
GACGCGT34100.064.0935751
AACCGCG39100.063.723111
CGCGTGA36650.063.609741
TCGCGAT24850.063.3046658
ATAACGC40450.062.677021
GTTACGC33150.062.32528
CGAACTA47700.061.5508841
CCGCTAT41650.061.221041
Produced by FastQC (version 0.11.5)