FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23040269
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2239535097.20090507623847No Hit
NGTCCAGA1739710.7550736495307411No Hit
GGTCCAGG531270.23058324536054678No Hit
GGTCCAGC480790.2086737789389525No Hit
GGGCCAGA422460.18335723424062453No Hit
GGTCCCGA304740.13226408077093196No Hit
GGCCCAGA265530.11524605029568014No Hit
GGTCCAGT252940.10978170437159393No Hit
TGTCCAGA240560.10440850321669422No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)