FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19108633
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA29100.082.476451
GTGCGAC31100.081.39471
TTACGCG11000.080.977469
CGCCGAA30550.080.4721761
ACGACCG28700.078.7964861
TTTGCGC31550.076.996661
CGGTACG29200.072.450631
GCGCTAG33400.071.4213941
GACGCGT30250.070.900451
TTACCCG37250.068.348061
CCGCTAT36350.066.026541
TCCGCGA25100.065.205838
AACGCGT29750.064.507733
GTACCGT41450.064.1199658
TAGGCGC34300.064.01761
GCGCGTA32750.063.9289551
CCTTACG36050.063.540621
GGCGACT37600.063.4434741
GCCGGAT38300.063.0458151
ATAACGC35450.062.1466521
Produced by FastQC (version 0.11.5)