FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16775467
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10850.084.188179
TGCGCGA27050.083.7246551
TTTGCGC28300.080.801021
CGGTACG26300.073.6120761
ACGACCG24800.073.056511
CGCCGAA26150.072.358081
GCGCGTA28500.070.7494661
GTGCGAC29500.070.084721
GCGCTAG28250.069.04811
TTACCCG31700.067.986341
CCTTACG32050.066.560071
CGCGGTA33550.065.5441
TCGTACG34050.064.937128
CGAACGC30650.064.833191
GACGCGT24750.063.758411
GTTACGC26450.063.313538
CCGCTAT32600.061.179231
ATAACGC32600.060.9551281
AACGCGT21100.060.8131073
GTACCGT38400.060.6015978
Produced by FastQC (version 0.11.5)