FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19724502
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG29950.084.387811
TTACGCG12700.077.633499
TGCGCGA29400.077.270471
TTTGCGC32400.075.301211
GTGCGAC34950.075.241221
CGCCGAA32400.074.85031
ACGACCG29650.074.401681
GCGCTAG32950.072.2707751
TAGGCGC37050.068.80781
CGAACGC35050.067.107081
TCGCGAT23500.066.6346058
GGCGACT37950.066.598551
GACGCGT30250.065.440171
TTCCGCG16800.065.160077
TCCGCGA28950.065.108658
TTACCCG37750.065.016381
TCACGCG27450.064.704978
TCGTACG40900.063.9878928
GTTACGC28550.063.7355168
CCTTACG38350.063.2372861
Produced by FastQC (version 0.11.5)