FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19724502
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT61200.0118.986166
ACGACGC61950.0118.479535
CACGACG64400.0114.53474
GACGCTC75000.096.802887
CTACACG79100.093.44291
TACACGA81100.091.2898562
ACGCTCT83350.087.45098
ACACGAC87450.084.6767353
CGCTCTT94900.076.96029
TCTTCCG144150.010.98763710-14
CTTCCGA159850.010.361732510-14
TTCCGAT198400.08.63325610-14
TCCGATC201100.08.22927615-19
CGATCTA48600.08.1401815-19
CCGATCT213500.07.98888515-19
GCTCTTC232700.07.068028510-14
CGATCTT71750.06.26105315-19
TAACCCT811450.05.68799836
CGTTGAG27551.8677893E-65.57826471
CTAACCC847100.05.5597325
Produced by FastQC (version 0.11.5)