FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19598502
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC59050.0118.099755
CGACGCT60000.0115.507276
CACGACG60750.0115.150834
GACGCTC73400.094.715937
CTACACG77200.091.2554551
TACACGA77150.091.229192
ACGCTCT80300.086.125818
ACACGAC82750.085.148633
CGCTCTT94600.072.800079
TCTTCCG138700.010.98610810-14
CTTCCGA160500.010.17140510-14
CGATCTA48250.09.04450915-19
TTCCGAT199900.08.32619810-14
TCCGATC204050.07.76603615-19
CCGATCT216700.07.620450515-19
CGCGCTT11953.8016489E-47.2796869
GCTCTTC228300.06.718887310-14
AACGGGG32656.6538632E-95.84379631
CGATCTT77000.05.648671615-19
TAACCCT807500.05.63793136
Produced by FastQC (version 0.11.5)