FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20332105
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1949528195.88422349776376No Hit
NAGTAGAG2054481.010461041785885No Hit
AAGGAGAG751140.3694354322879997No Hit
AAGTGGAG598480.29435220799813894No Hit
AAGTCGAG486500.23927674975119398No Hit
AAGTTGAG364430.1792386966327392No Hit
AGGTAGAG360150.17713365143451698No Hit
ACGTAGAG344070.16922497695147648RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGCG325370.16002770003401026No Hit
AAGTAGGG313680.15427817237811825No Hit
AAGAAGAG313160.15402241922319407No Hit
AAGCAGAG261850.12878646849403935No Hit
AAGTAGAA240480.11827599749263541No Hit
GAGTAGAG235010.11558567103602897No Hit
CAGTAGAG222110.10924102546194799No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)