FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20147831
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTTGAC1948904296.73022371490013No Hit
NACTTGAC1563520.7760239799509933No Hit
AGCTTGAC454790.22572653105934828No Hit
GACTTGAC423420.21015661685865838No Hit
CACTTGAC401520.1992869604673575No Hit
AACTTGCC305280.15152003210668186No Hit
TACTTGAC287350.14262081114339303No Hit
AACTTGGC271460.13473410611792408No Hit
AACTTGAA261030.12955737022014926No Hit
ACCTTGAC258190.12814778920867462No Hit
AACTGGAC243540.12087653504737061No Hit
AACGTGAC240830.11953147711036488TruSeq Adapter, Index 21 (100% over 8bp)
AACTTGAG239490.11886639311199304No Hit
AAGTTGAC224110.11123281707097901No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)