FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19026229
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23900.092.529136
ACGACGC26500.083.707695
CACGACG28350.079.011154
TACACGA37550.060.7140432
GACGCTC37950.058.848747
CTACACG44850.050.6175651
ACGCTCT44300.050.085828
ACACGAC53350.043.209143
CGCTCTT57400.039.034169
GTTCCGA51850.06.7109235
CGCTGAG39700.06.2717741
CGATGAG30601.0300937E-85.9830211
TAACCCT702000.05.845966
CTAACCC744100.05.6407535
TGTTCCG61950.05.61672074
CCTAACC768950.05.5526484
AACCCTA759400.05.54497051
ACCCTAA782700.05.49156142
CCCTAAC784350.05.48983763
AACGGAG37755.81349E-95.4317911
Produced by FastQC (version 0.11.5)