FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19234779
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24450.093.400296
ACGACGC25700.088.857015
CACGACG27500.083.568164
GACGCTC37250.061.8893977
TACACGA41150.057.282812
CTACACG45400.052.255161
ACGCTCT46300.049.9482848
ACACGAC53300.044.3413773
CGCTCTT63100.036.8803029
CGTTGGG35807.2759576E-126.32092051
TAACCCT733200.05.74473766
GTTCCGA50600.05.58763935
CTAACCC777250.05.57769445
AACCCTA787550.05.4778611
CCCTAAC810150.05.4765213
ACCCTAA813550.05.44712972
CCTAACC803900.05.41082534
TCTTCCG106750.05.406131310-14
CGATGAG31507.399485E-75.3298991
CGCTGAG40305.462425E-95.25284531
Produced by FastQC (version 0.11.5)