FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24137458
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2336352596.79364330742699No Hit
NGTCCAGA1864910.7726207125870503No Hit
GGGCCAGA576000.23863324795842214No Hit
GGTCCAGC444610.1841991812062397No Hit
GGTCCAGG392460.16259375780167074No Hit
GGTCCCGA370830.15363258218823206No Hit
GGTCGAGA362610.15022708687882544No Hit
GGTGCAGA352660.146104863237877No Hit
TGTCCAGA341300.14139848529203033No Hit
GGCCCAGA317210.13141814684876924No Hit
GGTCCGGA300250.12439172343666013No Hit
GGTTCAGA247710.10262472543711934Illumina DpnII expression Adapter 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)