FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16878178
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10200.082.4394159
TTTGCGC29800.080.9376751
TGCGCGA26700.076.647821
CGCCGAA27700.076.255481
ACGACCG25100.072.506781
CGGTACG27000.068.757961
GCGCTAG29050.068.183011
TTACCCG33300.067.163021
GGCGACT31900.066.902931
GTGCGAC30700.066.184981
CCGCTAT31950.065.883191
CGTTGCG13550.065.268757
ATAACGC30250.064.511761
GCGCGTA26800.063.8166581
GCCGGAT31900.063.695261
CGAACTA37850.063.530611
TCACGCG22000.062.9349148
GACGCGT27500.062.4580151
GTTACGC27400.062.4370548
CGCGGTA32000.061.897391
Produced by FastQC (version 0.11.5)