FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17745755
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11600.081.782989
CGCCGAA27800.081.596171
TTTGCGC27900.077.643731
ACGACCG28150.075.9177551
TGCGCGA28500.074.473591
GTGCGAC31200.074.3407061
GCGCTAG31050.072.585691
CGGTACG27800.071.626211
AACGCGT25600.070.722213
CGAACGC32300.067.970141
GGCGACT34300.066.5586241
TAGGCGC32000.066.1001051
GCGCGTA26600.065.808761
CCGCTAT34700.064.74041
CGCGGTA35700.064.35711
GTTCGCG37150.064.1337668
ATAACGC35650.064.038181
TTACCCG34600.063.8734971
GTTACGC28700.063.3346678
GTACCGT40500.062.5839048
Produced by FastQC (version 0.11.5)