FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17745755
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23600.096.434076
ACGACGC24100.095.937355
CACGACG26350.087.470284
GACGCTC36200.063.0686537
TACACGA39400.059.249382
CTACACG42100.056.0820121
ACGCTCT42700.053.807228
ACACGAC44100.053.2505573
CGCTCTT55600.041.323179
GTTCCGA46650.06.52546835
CGCTGAG35251.1568773E-95.8063351
TCTTCCG99650.05.79042310-14
TCGGTCC16400.00211558355.746234145
ACGGGAG42001.4551915E-115.74338341
CGTGGAG61800.05.44092231
TAACCCT470000.05.33569576
CTAACCC488100.05.2417945
TGTTCCG57050.05.2088554
CTTCCGA118250.05.173873410-14
CCCTAAC516900.05.14608863
Produced by FastQC (version 0.11.5)