FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18847963
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA27550.082.0620041
TTTGCGC30200.080.917921
TTACGCG13000.080.294949
CGCCGAA28800.075.705771
GTGCGAC34600.074.645531
CGGTACG28600.072.142011
ACGACCG28650.070.228481
ATAACGC34150.069.844481
GCGCTAG32600.069.3499451
TTACCCG35700.068.8614651
GCGCGTA29150.068.521881
GTTCGCG39250.068.518338
AACCGCG36850.066.712461
CGAACGC35250.066.004451
GTTACGC29450.065.473998
TCGTACG35750.065.088098
TCACGCG25750.065.029028
GGCGACT37250.064.424761
CCTTACG32900.063.6026651
TACGAAC43900.062.9988331
Produced by FastQC (version 0.11.5)