FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19941517
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12300.082.514589
TGCGCGA31750.077.468961
GTGCGAC37850.073.661251
TTTGCGC31300.073.452711
CGCCGAA30450.071.907731
ACGACCG29950.068.7216951
CGGTACG29850.068.4629061
CCGCTAT39800.067.668251
TAGGCGC38900.067.54521
GACGCGT29950.067.503231
GCGCGTA33900.067.388571
GTTACGC30300.067.231298
CGAACGC36000.067.1068951
GCGCTAG32600.066.493691
CGCGGTA38900.065.106071
TTACCCG37500.064.227981
ATTAGCG40550.064.076791
ACGCCGA38800.063.9571081
TAAACCG42400.063.690911
CGAACTA43650.063.3718761
Produced by FastQC (version 0.11.5)