FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17757414
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11250.079.259029
TGCGCGA27650.077.9214861
ACGACCG26900.076.835961
CGCCGAA28100.075.114171
TTTGCGC28250.074.715331
CGGTACG28650.072.9074251
CGAACGC33200.070.835041
GCGCGTA27800.069.88231
GCGCTAG32200.068.725371
CGTTGCG13950.068.595927
GTGCGAC31300.068.134771
ATAACGC32150.066.787731
GTTACGC26600.065.680128
GTTCGCG35750.065.497548
GGCGACT34850.064.966471
GACGCGT30600.064.919891
AACGCGT25200.064.738843
GTACGCT26300.064.22428
TCGTACG33700.063.6737378
TCCGCGA24950.063.6314938
Produced by FastQC (version 0.11.5)