FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17757414
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24850.0102.981366
ACGACGC25650.0101.1817865
CACGACG27850.093.448224
GACGCTC35550.071.9855657
TACACGA41350.063.996112
CTACACG44150.060.5016251
ACGCTCT43150.059.642788
ACACGAC46950.056.3565443
CGCTCTT56100.046.0035559
ACGGGAG41450.07.43568851
CGTAGAG27106.6029315E-106.76966331
TCTTCCG104250.05.994048610-14
CGTTGAG25803.3148026E-65.68861681
CTTCCGA122050.05.464357410-14
CTAACCC498600.05.29243765
ACCGGAG23601.6696073E-45.28607461
CGCCAAG28001.3916486E-55.2416541
TAACCCT485550.05.24062976
AACGGAT22956.384179E-45.1160371
CCTAACC523550.05.08170754
Produced by FastQC (version 0.11.5)