FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16902115
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT18650.083.881336
ACGACGC20450.077.914775
CACGACG22950.070.058474
GACGCTC28800.054.821947
TACACGA34350.046.4252172
ACGCTCT39100.041.1216438
ACACGAC44800.036.050253
CTACACG38250.034.800161
CGCTCTT51850.031.4289079
CGATGGA29650.08.9277781
CGTGATG46950.08.2155111
CGGATTA16352.9607654E-67.40120271
CGGGTTA28703.6379788E-127.3786381
CGATGGG33350.07.2569511
CGCTTAG14603.1185028E-46.73427061
CGAAAAG32255.638867E-116.56641531
CGGCAAT16201.5546127E-46.53601551
CGATGAG26801.36424205E-86.49072271
CGTAGAG25754.2344254E-86.4616811
CGCCAAT16401.8234074E-46.4563081
Produced by FastQC (version 0.11.5)