FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17194687
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10050.082.951259
TGCGCGA25250.077.736331
GTGCGAC28650.075.362181
CGCCGAA26500.074.892521
TTTGCGC27500.074.2841
ACGACCG25100.072.9874951
GACGCGT27250.069.36311
CGGTACG27650.069.148421
GCGCTAG31250.068.1615141
GCCGGAT34050.066.399511
GCGCGTA26700.066.163221
TTACCCG32250.065.8225561
CCGCTAT35000.065.635791
CGAACGC31950.064.3927841
TAGGCGC30750.064.30511
CCTTACG33900.063.90551
GGCGACT33000.063.8859981
ATAACGC31900.063.354251
GCGCAAC39600.062.784521
CGATCGG30300.062.3810041
Produced by FastQC (version 0.11.5)