FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17194687
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24100.096.771776
ACGACGC25200.092.8350145
CACGACG26450.087.35244
GACGCTC35750.065.641557
TACACGA39700.058.4313352
ACGCTCT41800.055.620978
CTACACG39150.049.6499061
ACACGAC47550.049.197773
CGCTCTT56250.041.204169
CGGGTTA26350.08.8981181
GTACACG22300.08.8183151
CGATGGA29150.07.78394561
CGCAAAG30050.07.55081561
CGTGATG45800.07.2661491
CGTAAAG22251.3522003E-76.79856251
CGTTGAA28851.364242E-96.55407331
TAACCCT683250.06.4981646
CGCCAAG27452.2320819E-86.33727551
TCTTCCG88050.06.333951510-14
CGCTATA14500.0021144026.25936561
Produced by FastQC (version 0.11.5)