FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16572583
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11050.086.595289
CGCCGAA28200.078.6451341
TGCGCGA23400.077.37571
ACGACCG25700.074.4121251
CGGTACG26050.072.2958151
GTGCGAC29000.071.7115551
TTTGCGC27500.071.128021
GCGCTAG30250.068.748271
CCGCTAT32000.068.624321
TTACCCG33600.068.386271
CGAACGC31000.066.381321
GTCGGAC25950.066.129581
GCGCGTA26150.065.901881
GTTACGC25050.065.4018258
ATAACGC33800.065.1849061
GACGCGT25850.064.697641
CGCGGTA34100.063.971711
GGCGACT33100.063.7075841
TCGTACG31100.063.634328
TTCCGCG16300.063.5971347
Produced by FastQC (version 0.11.5)