FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16572583
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT21200.096.3654566
ACGACGC22800.089.920985
CACGACG25050.081.847214
GACGCTC32650.062.5692527
TACACGA37150.055.795972
ACGCTCT41350.049.0534678
ACACGAC43050.048.4665263
CTACACG38800.048.401271
CGCTCTT53050.038.6450169
CGATGGA29100.08.8120111
CGTAGAG28652.3646862E-117.1076861
CGGACTA13157.222994E-46.88247441
CGATGGT36750.06.7724481
CGATGGG32658.185452E-116.467911
GTACACG20301.1088807E-56.3159981
CGGGTTG55300.06.2736721
GTTCCGA39500.06.2357875
TCTTCCG85700.06.15409910-14
CGCTAAG15000.00319100876.03363561
CTTCCGA95000.05.87193210-14
Produced by FastQC (version 0.11.5)