FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17686035
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10800.079.873579
TGCGCGA25950.079.573861
CGCCGAA28600.078.0480041
GCGCTAG30500.076.5234761
CGGTACG26750.074.204171
TTTGCGC26150.072.57021
ACGACCG24800.071.8296661
GTGCGAC30100.069.81051
TTACCCG33750.069.369981
GACGCGT27500.069.272051
GGCGACT34200.065.693421
CCGCTAT33450.065.210061
GTTACGC26400.065.0778058
CGAACGC33100.064.142271
ATAACGC34500.063.014651
GTTCGCG36650.062.8986938
TCGACGG31450.062.6524541
GTACCGT39100.062.294698
AACGCGT25150.061.9754453
TAGGCGC33450.061.9495541
Produced by FastQC (version 0.11.5)