FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17686035
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC23750.094.8645
CGACGCT23800.093.750216
CACGACG26400.085.6192254
GACGCTC34550.065.418817
TACACGA40250.057.08432
ACGCTCT41700.052.985248
ACACGAC46250.049.9685943
CTACACG41300.048.580921
CGCTCTT55500.040.2025689
CGAATCG5850.002286529210.3149781
CGTAGAG27950.09.4454011
CGTGATG45600.08.1052311
CGAAAAG32550.07.4153751
CGGGTTA26752.6557245E-107.04937121
GTACACG23809.336873E-96.9723611
CGGATTA17407.793251E-66.9359331
CGCTTAG15257.310632E-56.92456341
TAACCCT680650.06.63075166
CGATGGA30205.9117156E-106.4938251
CTAACCC724550.06.479055
Produced by FastQC (version 0.11.5)