FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16466568
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG9400.082.478859
TGCGCGA24550.077.881331
ACGACCG22500.077.545721
GTGCGAC27650.077.03741
CGCCGAA24200.075.4027941
TTTGCGC26900.075.1314241
CGGTACG26050.073.39681
GCGCGTA26600.071.332591
GCGCTAG28800.070.1748351
CGAACGC29950.069.4221951
GACGCGT24100.069.38091
GGCGACT33650.065.89371
TTACCCG30250.065.849771
TCGTACG32600.065.567868
AACCGCG32600.065.5631
CGTTGCG13400.065.436887
CGCGGTA32350.064.9460451
AACGCGT24100.064.650623
TAGGCGC33000.064.32771
ATAACGC33350.064.306561
Produced by FastQC (version 0.11.5)