FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15510187
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC21500.099.401915
CGACGCT22850.092.892956
CACGACG24450.087.7077564
GACGCTC33600.063.387127
TACACGA35700.061.1126022
ACACGAC39750.054.4951443
CTACACG35400.052.2015571
ACGCTCT41500.051.4948848
CGCTCTT52750.040.5128259
CGATGGA25050.08.4308421
CGGGTTA23701.8189894E-128.2745741
CGGATTA16906.2449726E-77.5872231
CGATGAG23801.2205419E-97.289061
CGCTGAG33850.07.13036871
CGTTGAG23003.572677E-86.8867151
CGTAGAG23605.8375008E-86.71162941
GTACACG21505.641323E-76.66554741
CGATGGG32557.4578566E-116.48825171
TCTTCCG80650.06.413625210-14
CTTCCGA88150.06.377481510-14
Produced by FastQC (version 0.11.5)