FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21184288
Sequences flagged as poor quality0
Sequence length8
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA1852898987.46571515644047No Hit
TGTCCAGA10340414.881169478058455No Hit
NGTCCAGA7119583.3607832370859008No Hit
AGTCCAGA2081590.982610319497167No Hit
CGTCCAGA1888800.8916041926922443No Hit
GGGCCAGA728450.34386333871593894No Hit
GGCCCAGA386490.18244181725626088No Hit
GGTCCAGC361260.17053204714739528No Hit
GGTCGAGA320820.15144242751986756No Hit
GGTCCAGG306800.14482431507728746No Hit
GGTCAAGA286300.13514733183385724No Hit
GGTCCGGA282000.13311752559255235No Hit
GGTCCCGA276770.1306487147455699No Hit
GGTGCAGA243510.11494839949305825No Hit
GGTTCAGA221320.1044736551919989Illumina DpnII expression Adapter 1 (100% over 8bp)
GGTCTAGA219390.10356260262322718No Hit
GGACCAGA214240.10113155561329228No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)