FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20963154
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA30200.081.382761
TTTGCGC34150.075.802221
GTGCGAC38000.075.585091
TTACGCG12550.073.32579
CGCCGAA31000.072.245641
ACGACCG30350.071.397051
GCGCTAG36600.069.337321
ATAACGC38150.066.52021
CGAACGC39600.065.73711
AACCGCG40000.064.897941
GGCGACT41200.064.596141
GTCGGAC30400.064.343011
CCGCTAT40950.063.9250761
CGAACTA40750.063.881941
GCCGGAT40000.063.8072171
CGGTACG33850.063.799961
TAAACCG47450.063.4434471
GACGCGT32850.063.3072361
TCGCGAT25300.062.721988
TTACCCG38100.062.408741
Produced by FastQC (version 0.11.5)