FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17307995
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT21550.086.094966
ACGACGC23800.079.47835
CACGACG24450.077.6618044
GACGCTC31100.060.5887157
TACACGA35500.054.1014442
CTACACG37200.046.6175421
ACGCTCT42000.045.554728
ACACGAC49000.039.0470163
CGCTCTT54450.035.1383259
CGATGGA32200.08.6638391
CGCTGAG35150.07.9367171
CGCAAAG29550.07.39954471
CGGATTA17801.4283996E-67.20100981
CGATGGG36900.06.9473161
CGGGTTA25505.1950337E-96.80067731
TAACCCT616150.06.422316
CGTAGAG28457.4196578E-96.36053231
CGCAAAT25302.191664E-76.2583991
CTAACCC657300.06.2297395
TCTTCCG74950.06.18854510-14
Produced by FastQC (version 0.11.5)