FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18513866
Sequences flagged as poor quality0
Sequence length8
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1647512788.98804279992089No Hit
NAGTAGAG6866903.7090578488577157No Hit
TAGTAGAG3144581.698499924326988No Hit
CAGTAGAG2743871.4820621473656557No Hit
GAGTAGAG2299641.242117664673602No Hit
AAGGAGAG1164430.6289502149361997No Hit
AAGTGGAG661900.3575158208447658No Hit
AAGTCGAG523450.2827340329675066No Hit
AAGTTGAG370580.20016348827414004No Hit
AAGAAGAG370080.1998934204233735No Hit
AAGCAGAG349890.18898808060942No Hit
AGGTAGAG318660.17211964265054094No Hit
AAGTAGGG279740.1510975611468723No Hit
AAGTAGCG269050.14532351049748335No Hit
ACGTAGAG212620.11484365285996992RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGAA194630.10512661158938927No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)