FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21302465
Sequences flagged as poor quality0
Sequence length8
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA1748438282.0768019100137No Hit
TGTCCAGA16837417.903972615375732No Hit
NGTCCAGA8473613.9777603202258516No Hit
AGTCCAGA4997062.3457660885723786No Hit
CGTCCAGA3945751.8522504320509388No Hit
GGGCCAGA515860.242159768834264No Hit
GNTCCAGA315960.14832086333670774No Hit
GGTCCCGA301950.14174415965476297No Hit
GGCCCAGA287240.134838855503342No Hit
GGTCCGGA286910.13468394385344606No Hit
GGTCCAGC246120.11553592506782667No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers