FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19833754
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGCCT1422419571.71710912618963No Hit
ATGAGCCT16344678.240835295224494No Hit
GTGAGCCT15465887.797757297988066No Hit
CTGAGCCT12289716.1963610116370305No Hit
NTGAGCCT8399884.2351437856897896No Hit
TTGAGCCG329290.16602505002330875No Hit
TTGAGCCC284820.14360367684302225No Hit
TTGGGCCT267360.13480050221455808No Hit
TGGAGCCT262240.13221904436245402No Hit
TTGAGGCT249540.12581581883086781No Hit
TTGAGCCA241300.12166128510013786No Hit
TTTAGCCT234210.11808657100415786No Hit
TTGAGCGT220820.11133545369172171No Hit
TTGCGCCT217010.10941448603224585No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)