FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15588676
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG24250.080.673531
TGCGCGA22500.080.4594651
TTTGCGC25600.076.134111
GTGCGAC25850.075.68021
CGGTACG24150.074.659971
CGCCGAA25300.072.9975051
TTACGCG8050.072.031049
ATAACGC29400.068.279971
GACGCGT22350.068.261641
GCGCTAG28050.067.662571
GCGCGTA25250.067.070971
TCGTACG29850.066.77658
TTACCCG30650.065.495311
CCTTACG28600.064.829951
TCCGCGG17700.064.702218
TCACGCG23100.064.638398
TCGACGG27950.063.9870761
CGAACGC30450.063.767931
CGCGGTA30800.063.7543071
GGCGACT30900.063.311751
Produced by FastQC (version 0.11.5)