FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15588676
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23900.079.400776
ACGACGC25800.076.081065
CACGACG28450.069.503614
GACGCTC33850.056.9163787
TACACGA40050.050.6841962
ACGCTCT41350.046.2511988
CTACACG38350.044.61531
ACACGAC48700.041.9419443
CGCTCTT56650.033.7596979
GTACACG23550.09.5179631
CGATGGA32751.6370905E-116.6160751
TCTTCCG78100.05.95490610-14
TAACCCT393100.05.93299776
CTTCCGA85350.05.90740310-14
CTAACCC417650.05.77508975
CCCTAAC437800.05.5920283
CGAAAAG40757.057679E-105.50056551
CCTAACC434550.05.50048734
AACCCTA428000.05.4998897
ACCCTAA445250.05.45453742
Produced by FastQC (version 0.11.5)