FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17600323
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA28550.077.922331
TTACGCG9550.076.663879
CGCCGAA29200.076.436751
CGGTACG28300.075.784821
TTTGCGC27850.073.615721
GTGCGAC32000.070.65731
GCGCGTA28400.070.398111
GCGCTAG31150.069.784641
GGCGACT34400.067.8411561
ACGACCG24900.067.15451
TTACCCG33200.066.351561
ATAACGC33850.065.507021
TAGGCGC33400.064.648231
TCCGCGA23300.064.096378
CCGCTAT33250.064.065271
GTACCGT41800.063.305098
AACGCGT25950.063.13873
CGGATCG37700.062.8670271
TTCCGCG16000.062.5294957
TACGAAC38800.062.2089731
Produced by FastQC (version 0.11.5)