FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18387752
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT17650.082.125196
ACGACGC20150.073.36755
CACGACG20450.071.936844
GACGCTC28350.050.8724757
TACACGA33050.046.489442
ACGCTCT37100.039.654788
CTACACG36450.036.6451531
ACACGAC46750.033.1748623
CGCTCTT50500.029.1322849
CGGGTTA30800.09.31051351
CGATGGA30550.09.1396871
CGACAAG19351.2514647E-98.189911
CGAAAAG32300.08.1772321
CGTAGAG28050.07.80199961
CGTTGAG24453.45608E-117.71617271
CGTGATG48200.07.67166851
CGCTAAT20101.733697E-77.13342861
CGCAAAG28652.3646862E-117.1118061
CGCCAAT16703.2528653E-56.77821871
CGGATTA18451.8392182E-56.54431771
Produced by FastQC (version 0.11.5)