FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15445020
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22050.097.606186
CACGACG24050.090.397274
ACGACGC24050.089.794625
GACGCTC32950.065.320397
TACACGA34500.064.949452
ACACGAC39550.056.435813
CTACACG35650.055.0679321
ACGCTCT41900.051.5418328
CGCTCTT50150.042.629769
CGTAGAG24202.7284841E-117.8002691
CGGATTA16302.8985542E-67.41169171
CGAAAAG29651.8189894E-127.3851321
CGTGATG39550.07.06382941
GTACACG19558.213228E-76.9520151
TCTTCCG74050.06.889656510-14
AACGGGG31957.2759576E-126.8534941
CGATGGA26158.4983185E-96.64111661
CTTCCGA82350.06.58245610-14
CGGGTTA23102.7536953E-76.53736831
TAACCCT519950.06.4806846
Produced by FastQC (version 0.11.5)