FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19827268
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12550.086.605739
TGCGCGA32750.078.1557541
CGCCGAA33750.077.132761
TTTGCGC32650.073.495441
CGGTACG29650.072.348071
GCGCTAG33250.069.544871
ACGACCG30100.069.0922241
GTGCGAC31850.068.035641
GTTACGC30550.067.5978248
TTACCCG37700.067.1224061
GACGCGT33100.064.807261
CGTTGCG16150.064.169947
TCGCGAT22500.063.764918
ATAACGC36450.063.6389161
CGAACGC41400.062.528681
AACCGCG33750.062.4818421
TCTACGG47950.062.108688
TCGTACG38700.061.974948
TAGGCGC38500.061.0058441
AACGCGT28100.060.865863
Produced by FastQC (version 0.11.5)